Figure 4

(a) Activity of pPAL, MSP-PAL, MSP-PAL coated with PAH and PAA-BB (MSP-PAL_PPB) in SIF at 37 °C. (b) Activity of pPAL, MSP-PAL, MSP-PAL coated with PAH and PAA (MSP-PAL_PP) and MSP-PAL_PPB in SIF (dotted lines) and in the presence of trypsin 0.4 mg/mL (solid lines) at 37 °C. Data represent the mean ± SD (n = 5), two-ways ANOVA (*p < 0.05) (a) between pPAL and MSP-PAL_PPB, (b) between trypsin treated MSP-PAL_PPB and trypsin treated MSP-PAL_PP (*p < 0.05).