Figure 3
From: Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis
Spatio-temporal analysis of retinoid fluorescence (RF) and collagen fibers (CF/SHG). (a) Example of collocation of retinoid fluorescence (RF) imaged by fluorescence (left) and collagen fibers imaged by second harmonic generation (CF/SHG) (middle) microscopy. Right: merge of RF and CF/SHG signals. (b) Percentage of RF signal surface area on total SD- or CDAHFD-liver slices as a function of time. (c) RF patches density (number of RF patches per mm²) of total SD- or CDAHFD-liver slices as a function of time. (d) Percentage of CF/SHG signal surface area on total SD- or CDAHFD-liver slices as a function of time. (b–d) Statistics: n = 3–12 for each group, median with interquartile range, Kruskal Wallis test. (e) Merge of RF and CF/SHG signals of 9 weeks CDAHFD-fed mouse liver slice (left). Heat map of RF patches density of the same liver slice (right). Color scale = number of RF patches for a 100 × 100 µm square. PV = Portal Vein, CV = Centrilobular Vein. (f) Total liver content in retinyl esters measured by HPLC as a function of time of SD or CDAHFD. (n = 4–7 for each condition, means ± standard deviation, Mann-Whitney test). *p < 0.05; **p < 0.01; ***p < 0.001.
