Figure 1

Characterisation of carbohydrate metabolism in equine COCs Data describing glucose consumption (BLUE) and lactate production rates (RED) in equine COCs during in vitro maturation (n = 29). Panels (A,D) denote the mean (±SEM) rates for individual COC and are presented per COC/h (A) and per ng DNA/h (D). Panel (B) shows linear correlation between glucose consumption and lactate production rates (r² = 0.59; p < 0.001). COCs that depleted all available glucose are highlighted separately (aqua [glucose] and orange [lactate]). Panel (C) shows correlation of both glucose consumption and lactate production with DNA content (r² = 0.3; P = 0.01). Glucose consumption and lactate production rates by maturation status (E; metaphase II [MII] and degenerated [DEG] oocytes) and cumulus classification (F; compact [CP] and expanded [EX]). Bars represent mean ± SEM. (n = 29). There were no statistically significant differences between either maturation status or cumulus classification (p > 0.1).