Figure 3
From: Energy metabolism of the equine cumulus oocyte complex during in vitro maturation
Oxidative metabolism of COCs during IVM over time. Mean (± SEM) Basal O2 consumption rates (OCR) measured at 4, 12 and 28 h after the initiation of the 30 h in vitro maturation period (A; IVM +4, +12, +28) (n = 12 COCs). Mitochondrial function at IVM + 4 h (outset) and IVM + 28 h (end) as determined from respiratory inhibitor studies (B; n = 57 COCs). OCR coupled to ATP production (BLUE), OCR associated with proton leak (RED), non-mitochondrial OCR (GREEN) and spare respiratory capacity (PURPLE) are expressed as % basal OCR. Mean values are stacked. There was a significant difference between spare capacity at time IVM +4 and IVM +28 h; P<0.01.
