Figure 4
From: Energy metabolism of the equine cumulus oocyte complex during in vitro maturation
The effect of 0.15 mM pyruvate and O2 concentration on carbohydrate and oxidative metabolism of COCs during IVM. The effect of 0.15 mM pyruvate and O2 concentration (5% vs. 21%) on glucose consumption (A), lactate production (B), lactate:glucose ratio (C) and pyruvate production (D) during IVM. Condition abbreviations on X axes are as follows; 21C = Control maturation medium and maturation at 5% CO2 in air (21% O2); 21 P = Pyruvate containing Maturation Medium (control medium as above with the addition of 0.15 mM Pyruvate) and incubation at 5% Co2 in air (21% O2); 5C = Control maturation medium and maturation at 5% CO2, 5% O2 and 90% N2; 5 P = Pyruvate containing maturation medium and incubation at 5% CO2, 5% O2 and 90% N2. Bars represent mean ± SEM and different superscripts denote significance differences between groups (P < 0.05). Effect of medium pyruvate concentration (0, 0.15, 0.5 mM) during IVM on basal OCR (E; n = 45). Mean ±SEM are presented. Effect of pyruvate concentration during IVM on mitochondrial function as determined following the sequential addition of respiratory inhibitors (F; n = 45). O2 consumption rate (OCR) coupled to ATP production (BLUE), OCR associated with proton leak (RED), non-mitochondrial OCR (GREEN) and spare respiratory capacity (PURPLE) are expressed as % basal OCR. Mean values are stacked and there were no significant differences between groups.
