Figure 10

Homoeologous-specific expression of the chromosome I cytoplasmic TaCLPBs in four wheat genotypes under different heat treatments. Transcript analysis was done by qPCR using a relative quantification in three biological replicates. 2dct represents a normalized expression ratio (in comparison with actin reference), in the leaves of 12-day old seedlings under 22 °C control (A), and heat treatments of 2 h at 37 °C; 4 h at 37 °C; 2 h 37 °C plus 4 h at 42 °C (B). 2ddct represents a calibrated expression ratio of transcripts between a treatment and the control. Statistics were done using the analysis of variance (anova) for multiway-treatment structure, followed by a post-hoc multiple comparison using Tukey’s test. Letters are indicating statistical variation among genes at the control. Stars are representing significant changes of expression in comparison with the control (Significance codes: <0.0001 ‘***’; <0.001 ‘**’; <0.01 ‘*’; <0.05 ‘ . ’).