Figure 7
From: Chemokine releasing particle implants for trapping circulating prostate cancer cells
Reduction of cancer metastasis via cancer trap. Biodistribution of KD cells treated with the EPO-loaded particles or untreated control was quantified based on the measurement of organ-specific fluorescent intensities. To observe the biodistribution, NIR-labeled cancer cells were administered intravenously 12 hours following subcutaneous particles implantation injection or no treatment. (A) NIR imaging of internal organs were imaged 36 hours after cell administration. (B) A depiction of the organ arrangement. (C) The percentages of the cell distribution in different organs were calculated based on the individual organ fluorescent intensity divided by total internal organ fluorescent intensities. (D) Histological images of metastatic lung showed the accumulation of KD cells in lung (400X magnification). Scale bar: 50 µm (E) The lung tissue sections were used to quantify DiD+ cancer cells in lung sections from animals with EPO-loaded particles (EPO + HA) (829 ± 429.6) vs. untreated control (Control) (44 ± 49.8) groups. (n = 4) Data are mean ± SD. (Student’s t-test, *p < 0.05) Mesenteric lymph nodes is abbreviated to mLN.
