Figure 6

Absence of TTR increases the migration of OPCs isolated from the cerebral cortices of E18 and P7 mice. (A) The migratory rates of TTR null OPCs compared to wild type OPCs were analyzed using the modified Boyden chamber assay (migration assay). Representative photomicrograph high power fields (HPF = 0.3 mm²) (400X) of migrated OPCs isolated from cortices of E18 and P7 wild type and TTR null mice were stained with crystal violet. (B) Data were analyzed from 3 independent experiments for the E18 group and 5 independent experiments for the P7 group. All data were expressed as the mean ± SEM. Statistical comparisons were performed using a one-way ANOVA with post hoc analysis using Sidak’s test as appropriate (P < 0.05 was considered statistically significant)³⁰.