Table 1 Media used for NSC differentiation assays.
From: The Role of Transthyretin in Oligodendrocyte Development
Medium type | Component | Company | Final concentration |
|---|---|---|---|
NSC/OPC proliferation complete medium | KnockOutâ„¢ D-MEM/F-12 | Invitrogen | 1X |
StemPro® Neural Supplement | Invitrogen | 2% | |
GlutaMAX™-I Supplement | Invitrogen | 2 mM | |
bFGF (100 μg/ml stock) | Invitrogen | 20 ng/ml | |
EGF (100 μg/ml stock) | Invitrogen | 20 ng/ml | |
Antibiotic-Antimycotic | Invitrogen | 1X | |
NSC differentiation medium | Neurobasal® Medium | Invitrogen | 1X |
GlutaMAX™-I Supplement | Invitrogen | 2 mM | |
B-27® Serum-Free Supplement | Invitrogen | 2% | |
Antibiotic-Antimycotic | Invitrogen | 1X | |
Astrocyte differentiation medium | D-MEM | Invitrogen | 1X |
N-2 Supplement | Invitrogen | 1% | |
GlutaMAX™-I Supplement | Invitrogen | 2 mM | |
FBS | Invitrogen | 1X | |
Antibiotic-Antimycotic | Invitrogen | 1X | |
Oligodendrocyte differentiation medium | Neurobasal® Medium | Invitrogen | 1X |
GlutaMAX™-I Supplement | Invitrogen | 2 mM | |
B-27® Serum-Free Supplement | Invitrogen | 2% | |
T3 | Sigma | 30 ng/ml | |
Antibiotic-Antimycotic | Invitrogen | 1X |
