Figure 2
From: Neuronal contact guidance and YAP signaling on ultra-small nanogratings

Neurite alignment along NGs. (a) Bright-field images of PC12 neuronal cells on T1, T600, T400, T200 and Flat, at 24 h; the arrows indicate the NG direction; scale bar = 10 μm. (b) Neurite alignment on NGs. ***P < 0.001 (One-way ANOVA, Tukey’s test); at least 300 cells –at least 450 neurites- were analyzed for each substrate and for each NG type we performed n ≥ 6 independent experiments. (c) Representative scanning electron microscopy (SEM) images of PC12 neurites on T1, T600, T400 and T200; scale bars = 5 μm.