Figure 7
From: Neuronal contact guidance and YAP signaling on ultra-small nanogratings
Activation of the YAP/TAZ pathway in PC12 cells differentiating on ultra-small NGs. (a) Representative Western-blot panels (from the same gel; full-length gels are included in Fig. S3) and blot analysis of YAP 1 expression levels. Results (normalized to GAPDH levels) were reported in % (with respect to T1 levels; n ≥ 3. (b) Confocal representative images of YAP (green, first column) and actin fibers (red) with nuclear (blue) staining in PC12 cells on different substrates; scale bars = 10 μm. (c) YAP intracellular localization: YAP activation is reported as YAP nuclear/cytoplasmic ratio on different substrates; at least 25 cells were analyzed for each sample (n ≥ 3). (d) YAP nuclear/cytoplasmic ratio on all NGs- pooled together and Flat surfaces: #P < 0.01 NGs vs. Flat (Student’s t-test).
