Figure 8
From: Neuronal contact guidance and YAP signaling on ultra-small nanogratings
Impact of ultra-small NGs on hippocampal primary neurons (HNs). (a) Bright-field images of HNs from wild-type mice on T1, T600, T400, T200 and Flat, at day-in-vitro (div) 2; the arrows indicate the NG direction; scale bar = 25 μm. (b) HNs network development on ultra-small NGs: we quantified the % of HNs aligned network- i.e. the sum of all neurites with angle 0–15° vs. NG direction (squared columns)- and the % of perpendicular network- i.e. with angle 75–90° vs. NG direction (striped columns)- over the total neuronal network analyzed. (c) Neurite alignment angle of HNs on ultra-small NGs. *P < 0.05 T1 vs. T200, **P < 0.01 T1 vs. Flat (One-way ANOVA, Tukey’s test). We performed n = 4 independent experiments for T1, and n = 2 independent experiments for T600-T400-T200, with at least 15 neurons analyzed for each substrate; data = mean ± SD.
