Figure 2
From: Allosteric inhibitor of β-catenin selectively targets oncogenic Wnt signaling in colon cancer
Selectivity of C2 for Wnt pathway. (A) Screening of β-catenin-dependent cell lines. (B) Effect of C2 on viability of colon cancer cell lines (24 hour treatment). IC50 values are shown for respective cell lines. (C) Colony assay for WNT-dependent vs independent cells (7 day treatment). The number of colonies in each well was counted after 7 days of incubation. (D) Cancer 10-pathway selectivity assay (24 hour treatment). (E) Western blot analysis of Wnt activity in DLD1 and SW480 cells (24 hour treatment). All measurements were done in triplicates (n = 3).
