Figure 3
From: Allosteric inhibitor of β-catenin selectively targets oncogenic Wnt signaling in colon cancer
Mechanism of action of C2. (A) Time-course analysis of phosphorylation of β-catenin (1 µM C2). (B) Effect of C2 on ubiquitination of β-catenin (3 hour treatment). MG132 was used as proteasome inhibitor. (C) Combination of C2 (1 µM) with GSK3β inhibitor CHIR99021 (1 µM) (3 hour treatment). (D) Cellular localization of β-catenin as measured by confocal microscopy (24 hour treatment). Co-localization factor represents the level of β-catenin overlapping with nuclear DNA. Co-localization factor was calculated as 0.62 ± 0.03 and 0.25 ± 0.15 for DMSO and C2 treatment, respectively. (E) Nuclear-cytoplasmic fractionation of cells treated with C2. All measurements were done in triplicates (n = 3).
