Figure 2 | Scientific Reports

Figure 2

From: Carbohydrate response element binding protein (ChREBP) correlates with colon cancer progression and contributes to cell proliferation

Figure 2

ChREBP knockdown inhibited glycolysis, lipogenesis and p21 in HT29 cells. (A) Inhibited relative mRNA expression of ChREBP α and ChREBP β normalized to B2M after control or ChREBP siRNA transfection for 48 hours. (B) Western-blot of ChREBP, phospho-p53 and p21 and their quantification on the right. β-actin was served as a loading control. Proteins were extracted from cells transfected with sicontrol and siChREBP after 48 hours. The quantification of western blot was normalized to β-actin. (C) Decreased relative mRNA expression of glycolytic and lipogenic genes, normalized to B2M. (D) Relative mRNA expression of p53 and p21, normalized to B2M. (E) Relative mRNA expression of cell Cyclins, normalized to B2M. n = 4. *p < 0.05; **p < 0.01; ***p < 0.001.

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