Figure 4 | Scientific Reports

Figure 4

From: Carbohydrate response element binding protein (ChREBP) correlates with colon cancer progression and contributes to cell proliferation

Figure 4

ChREBP knockdown inhibited HT29 proliferation and induced cell cycle arrest. (A) Crystal violate staining of HT29 cells after being transfected with or without siRNA for 2 days, 4 days and 6 days. No treatment, no siRNA was transfected in HT29 cells. siControl, non-targeting siRNA; siChREBP, siRNA targeting ChREBP. (B) Quantification of the crystal violate staining, indicating inhibited cell proliferation after being transfected with ChREBP siRNA for 2 days, 4 days and 6 days. (C) Cell apoptosis assessment with flow cytometry after treatment with or without siRNA for 48 hours, showing no changes after ChREBP knockdown. Lower right corner represents early apoptosis, while upper right corner represents late apoptosis or cell death. (D) Cell cycle measurement with flow cytometry after treatment with or without siRNA for 48 hours, showing cell cycle arrest after ChREBP inhibition. All assessments were repeated 3 times. n = 4. *p < 0.05.

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