Figure 2

P.g.-odontogenic infection promotes liver fibrosis in model mice. The liver in HFD group (a) and HP group (b) were histologically analyzed including H&E staining (Magnification x100) and immunohistochemistry. Expression of Gal-3 and pSmad2 were analyzed immunohistochemically. Gal-3; Arrow: hCLS. Arrowhead: Gal-3 expressing hepatocyte. pSmad2; Arrow: HSC. Arrowhead: hepatocyte. Magnification: X200. (c) P.g. was detected in hepatocytes with P.g.-odontogenic infection. Arrow: P.g. Magnification: X1000. (d) hCLS was immunohistochemically detected as accumulation of Gal-3-positive macrophages. Magnification: X200. The number of hCLS was counted and number/unit area was calculated. (e) The red-stained fibrosis area was measured, and area/unit area was calculated. Magnification: X200. HFD = High Fat Diet (N = 5), HP = HFD + P.g.-infection (N = 5). Results were shown as mean ± SD. *p < 0.05, **p < 0.01. Gal-3: Galectin-3.