Figure 1
From: Verifying molecular clusters by 2-color localization microscopy and significance testing
Analysis of localization maps with 2-CLASTA. (a) Simulated two-color localization maps for a random (left column) and a clustered (right column) distribution of biomolecules. Localization maps show a 2 × 2 µm2 region. For the simulation of blinking we used experimental data obtained for SNAP488 (blue channel) and SNAP647 (red channel). (b) Shifting all localizations of the blue color channel by the shift vector \(\overrightarrow{v}\) breaks correlations between the two color channels. (c) The cumulative distribution function of nearest neighbor distances, r, between the two color channels is plotted in green for the localization data shown in (a). \(cd{f}_{rand}\) of N = 99 control curves, generated with randomly chosen toroidal shifts, are depicted in light gray. The mean of all control curves is shown in black. From the rank of the curves, we calculated a p-value of p = 0.50 for the random case, and p = 0.01 for the clustered case.
