Figure 2
From: Verifying molecular clusters by 2-color localization microscopy and significance testing
Robustness of 2-CLASTA for the detection of different degrees of oligomerization. To assess the influence of individual parameters we determined the sensitivity as a function of the number of molecules (a), the labeling efficiency (b), the labeling ratio (c), and directional stage drift (d). We simulated dimers (+), trimers (▲) and tetramers (■), both for the “ideal” (solid line) and the “realistic” scenario (dashed line). For panel (d), virtually all simulated scenarios yielded a sensitivity of 1. If not varied in the respective subpanel, parameters in all simulations were set to a molecular density of 75 molecules/µm2, a labeling efficiency of 40% for the real case and 100% for the ideal case, a labeling ratio of 1:1, and no stage drift. Each data point corresponds to 100 independent simulations.
