Figure 3
From: Verifying molecular clusters by 2-color localization microscopy and significance testing
Sensitivity of 2-CLASTA to detect protein enrichment or depletion. (a) We determined the sensitivity of 2-CLASTA for varying densities of circular domains and percentage of molecules inside the domains. Data are shown for a cluster radius of 100 nm for the “ideal” case and the “realistic” case (see Fig. S8 & S9 for other cluster radii). (b) The sensitivity for the detection of rectangular clusters with a size of 80 × 400 nm2 is shown for the ideal case and the “realistic” case. Numbers in individual fields indicate the average number of molecules per domain, and the relative enrichment or depletion of molecules compared to a random distribution with identical average density. The gray sale indicates the fraction of scenarios with a p-value below the significance level α = 0.05, reflecting the sensitivity. Each field corresponds to 100 independent simulations.
