Figure 4
From: Verifying molecular clusters by 2-color localization microscopy and significance testing
2-CLASTA analysis of an experimental data set. We analyzed GPI-anchored concatemers of SNAP-tags with n = 1 to 4 subunits expressed in HeLa cells as mimicry of n-mers. For dSTORM experiments, cells were labeled with SNAP488 and SNAP647. Panels (a) show two-color localization maps for representative cells, and panels (b) histograms of p-values obtained from at least 4 independent experiments per n-mer. Scale bars 250 nm (inset) and 2 µm.
