Figure 1
From: A quiescent state following mild sensory arousal in Caenorhabditis elegans is potentiated by stress
Mechanosensory stimulus during UV SIS elicits post response quiescence. (a) (top) Fraction of time quiescent of WT animals after 1000 J/m2 UV (blue) and non-exposed controls (black) in the absence of mechanostimuli. Dots represent individual animal quiescence in 1 or 2 hour bins. n = 15 animals per condition, four replicates. **Denotes p < 0.01 comparing UV-treated to controls (Wilcoxon rank-sum test with Bonferroni correction for five comparisons). (bottom) Quantification of PRQ. Change fraction in quiescent (∆ quiescence) is the peak quiescence within two minutes after mechanostimulus minus the peak quiescence within two minutes before mechanostimulus in the time bin specified. Typically, four or eight responses were averaged per animal and smoothed with a 10 s averaging filter. n = 22 animals per condition, two replicates. **Denotes p < 0.01, *denotes p < 0.05 (two-sided Wilcoxon signed rank test with Bonferroni correction for five comparisons). Error bars indicate ± SEM. (b) Mechanosensory response during UV SIS normalized to non-UV controls. Response is the average activity from the five seconds after the stimulus minus the average activity in the minute before the stimulus. The data are from the same animals as panel a (bottom). **Denotes significance (two-sided Wilcoxon rank-sum test with α = 0.01 and Bonferroni correction for five comparisons). Error bars indicate SEM. (c) Fraction quiescent before and after stimulus (dashed red line) 0–1, 1–2, 2–4, 4–6, and 6–8 h (blue) after UV treatment and in non-UV controls averaged throughout the experiment (black). Data are from the same animals as panel a (bottom) and panel b. Shading denotes ± SEM. (d) Schematic of post-response quiescence: (1) following stimulus, (2) the animal responds by moving, and (3) subsequently becomes quiescent.
