Figure 2

PRQ occurs after EGF overexpression. (a) (top) Fraction of time quiescent of hsp:EGF (red, circles) and WT (black, circles) after a mild, 10 min, 32 °C, heat shock and hsp:EGF held at room temperature (burgundy, squares) in the absence of mechanostimulation. n = 8 to 11 animals per condition, two replicates. **Denotes p < 0.01 comparing heat shocked hsp:EGF to WT controls (two-sided Wilcoxon rank-sum test with Bonferroni correction for six comparisons). (bottom) Quantification of PRQ in hsp:EGF (red) and WT controls (black) after mild heat shock. ∆ quiescence was calculated as in Fig. 1a. Pale markers represent individual animal averages. **Denotes p < 0.01, two-sided Wilcoxon signed rank test with Bonferroni correction for six comparisons. Error bars indicate ± SEM. (b) Mechanosensory response after EGF overexpression normalized to mildly heat-shocked WT controls. Response is the average activity from the five seconds after the stimulus minus the average activity in the minute before the stimulus. n = 28, four replicates for hsp:EGF and n = 10, two replicates for WT controls. *Denotes p < 0.05, **denotes p < 0.01, two-sided Wilcoxon rank-sum test with Bonferroni correction for six comparisons. Error bars indicate SEM. (c) Fraction quiescent before and after stimulus (dashed red vertical line) in hsp:EGF animals 0–1 h,1–2 h, 2–4.5 h, 4.5–7 h, 7–9.5 h, and 9.5–12 h after heat shock (red) and WT animals 0–12 h after heat shock (black). Shading denotes ± SEM. The animals are the same as those in panel b.