Figure 3

PRQ assays during ethanol, high salt, Cry5b toxin, heat, and cold – induced SIS. PRQ following mechanostimulus after various stressors and EGF overexpression (black, circles) compared to experiment-specific negative controls (gray, squares). (left) Comparison of PRQ after 15 min immersion in 5% ethanol by volume (purple, triangles), +500 mM NaCl in NGMB (yellow, diamonds), and EGF overexpression (black, circles) to NGMB-immersed controls (gray, squares). (middle) Comparison of PRQ after 15 min exposure to Cry5b-expressing bacteria (green, diamonds) or EGF overexpression (black, circles) to empty vector (EV) bacteria-exposed animals (gray, squares). (right) Comparison of PRQ after 30 min, 36 °C heat shock (orange, triangles) or 24 h, 4 °C cold shock (blue, diamonds) to room temperature controls (gray, squares). Pale markers represent individual animal averages over the specified time period. ∆ quiescence was calculated as in Fig. 1a. Error bars indicate ± SEM. *Denotes p < 0.05 and **denotes p < 0.01 in a two-sided Wilcoxon rank-sum test after Bonferroni correction for twelve (liquids, left; temperature, right) or eight (Cry5b toxin, middle) comparisons.