Figure 4

The ALA and RIS neurons and neuropeptide signaling are required for PRQ. (a) Fraction of time quiescent of WT (black), apft-1 (red), and ceh-17 (blue) mutant animals following EGF overexpression in the absence of additional stimuli (quiescence averaged in 1 h bins, n = 8 animals per genotype, one replicate). Error bars represent ± SEM. (b) Fraction quiescent before and after mechanostimulus (vertical dashed line) in WT (black) and aptf-1 (red) and ceh-17 (blue) mutant background animals carrying the hsp:EGF array 2–12 h after mild heat shock. n = 16 animals per genotype, two replicates. Shading represents ± SEM. (c) (left) Quantification of PRQ in WT and aptf-1 and ceh-17 mutants in panel b and (right) ceh-14, egl-21, and unc-31 mutants. ∆ quiescence was calculated as in Fig. 1a. Dots represent individual animals. n = 12 animals, two replicates for ceh-14; n = 24 animals, two replicates for egl-21; and n = 36 animals, three replicates for unc-31. **Denotes p < 0.01 (two-sided Wilcoxon signed-rank test with Bonferroni correction for three comparisons, left, one sample two-tailed t-test, no correction, right). Error bars represent ± SEM.