Figure 1

Multiple alignment of the precursor sequences of sea anemone Kunitz-type peptides. InhVJ20,49, APHC119,48, HCIQ1c9-HCIQ6c38, HCRG2c8, HCRG2c10, HCGS2c2, HCGS2c4 from H. crispa; HMIQ3c1 from H. magnifica13; SHPI-1 from Stichodactyla helianthus54. The asterisks (*) below the sequences indicate the contact sites with serine proteinases. Р1 – amino acid residue of the inhibitor reactive center. Signal peptide is shown in italic. The pro-part is underlined. Identical and conservative residues are shown in light and dark grey respectively. Horizontal arrows indicate the primer directions.