Table 2 Critical comparison of the proposed method with reported methods for determination of MDA.
Method | Reagent | Reaction conditions | Linear range (nmol/L) | LOD (nmol/L) | Recovery (%) | Ref |
|---|---|---|---|---|---|---|
UV-vis spectrophotometry | TBA | 100 °C, 60 min | 1000–5000 | 80 | 66–71 | |
Fluorescence spectrophotometry | TBA | 90 °C, 60 min | 14–1400 | 4.51 | 95.5–96.8 | |
HPLC-UV | TBA | 100 °C, 15 min | 86–9100 | 34 | 90–94 | |
HPLC-UV | DNPH | Room temperature, 30 min | 200–20000 | 200 | 96.3–99.8 | |
HPLC-UV | DAN | 37 °C, 180 min | 76–608 | <50 | 93–108 | |
HPLC-FLD | FMOC- hydrazine | 50 °C, 4 h | 500–10000 | 4 | 90.2–100.4 | |
HPLC-FLD | TBA | 100 °C, 60 min | 150–2430 | 15 | 100.4 | |
HPLC-FLD | AHAD | 80 °C, 30 min | 2–2500 | 0.25 | 95.7–101.2 | This work |
