Figure 6
From: Discovery of a bacterial peptide as a modulator of GLP-1 and metabolic disease
The role of Hld on calcium signalling. (A) Fluorescence microscopy imaging of intracellular calcium flux using HEK 293 GCaMP6S cells exposed to HldSe and HldSa. (B) Single cell calcium traces for five representative cells treated with HldSe (left) or HldSa (right). GCaMP6s is shown as relative fluorescence (F/F0). (C) Quantification of the maximum increase in GCaMP6s fluorescence. **p < 0.01 by Kruskal-Wallis One-way ANOVA. (D) Quantification of the area under the curve (AUC) for GCaMP6s fluorescence in cells treated with HldSe or HldSa. **p < 0.01 by Mann Whitney t test. (E) Patch clamp of NCI H716 cells to measure membrane depolarization for untreated cells or cells treated with HldSe or HldSa peptides (**p < 0.01 by ordinary One-way ANOVA).
