Figure 1
Dasatinib decreases viable cells and induced cell cycle arrest of EBV-LCLs. (A) The LCLs were cultured at the density of 2 × 104 cells/mL with indicated doses of dasatinib for 72 hours (n = 3), and CellTiter-Glo assay was performed. The relative intensity of luminescence to that of a negative control (0 μM dasatinib) was calculated. (B,C) The LCLs were cultured at the density of 1 × 105 cells/mL with indicated doses of dasatinib for 24, 48, and 72 hours (n = 3). Subsequently, the cells were fixed with in 70% ethanol with RNase, stained with PI, and analyzed by flow cytometry. (B) Gating strategy (upper) and representative plots (bottom) after 24 hours culture are shown. (C) Graphs indicates mean ± SD of the proportion of cells in G0/G1, S, G2/M phase after 24, 48, and 72 hours culture. The graphs are representatives of two independent experiments. Statistical analysis was performed using one-way ANOVA and subsequent Tukey’s HSD method. Values not sharing a common letter are significantly different (p < 0.05) shown in Fig. 1A and S1.
