Figure 2 | Scientific Reports

Figure 2

From: Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array

Figure 2

ChETA-mediated optogenetic modification of HEK293T cells and experimental approaches for minimizing optical interference. (a) Schematic illustration of the preparation of ChETA-expressing HEK293T cell. (b) Schematic illustration of membrane-inserted ChETA and enhanced yellow fluorescence protein (EYFP). Illumination of blue light induces the influx of cations via ChETA. (c) Confocal microscope images of ChETA-expressing HEK293T cells. (d) Confocal microscope image of ChETA-expressing HEK293T cells seated on a VNMEA pad. Scale bar, 20 μm. (e) Whole-cell patch clamp recording of ChETA-expressing HEK293T cells. (f) Schematics of solutions for mitigating the generation of light-induced artifact by minimizing the exposed metal (Pt) surface of electrodes.

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