Table 2 Physicochemical characteristics of the purified MNPs/PLL100-PEG/SOD1 complexes at various charge ratiosa.

From: Enzyme Release from Polyion Complex by Extremely Low Frequency Magnetic Field

Molar ratio of PLL amino groups to SOD1 carboxyl groups during the complex formation

2:1

1:1

1:2

DHD, nm

110 ± 12

116 ± 6

104 ± 8

PDI

0.22

0.37

0.26

ξ-potential, mV

27 ± 5

30 ± 7

25 ± 8

SOD1/MNPs ratio, µg SOD1/µg Fe3O4

0.281 ± 0.014

0.294 ± 0.015

0.228 ± 0.014

  1. aThe DLS measurements were carried out at RT in 0.1 mM HEPES buffer, pH 7.4. For all samples the Fe3O4 concentration of was 10 µg/mL. The SOD1 and Fe3O4 contents were determined by ICP-MS.
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