Table 1 Identifications of electrophysiologically active peaks by coupled gas chromatography–electrophysiology (GC-EAG-active) in a volatile extract from bed bug-exposed paper.

GC-EAG-active peak	Retention Index (HP-1)	Retention Index (DB-WAX)	Chemical	Extract concentration ng/µl (n = 3)	EAG responses			Blend composition
GC-EAG-active peak	Retention Index (HP-1)	Retention Index (DB-WAX)	Chemical	Extract concentration ng/µl (n = 3)	Males n = 10	Females n = 10	Total (n = 20)	SB-6	SB-16	SB-18
1	n/c	5.84	Hexanal	14.61	5	4	9	•	•	•
2	880	7.38	Heptanal	4.92	6	7	13	•	•	•
3	933	8.12	Benzaldehyde	4.84	5	3	8		•	•
4	965	8.56	(RS)-1-Octen-3-ol	25.95	4	7	11		•	•
5	983	8.81	Octanal	73.42	9	10	19	•	•	•
6	1000	9.06	3-Carene	28.03	4	7	11		•	•
7	1023	9.34	β-Phellandrene	28.98	5	6	11		•	•
8	1036	9.50	(E)-2-Octenal	102.23	8	8	16	•	•	•
9	1068	9.90	(3E,5E)-Octadien-2-one	43.35	8	5	13			•
10	1078	10.03	Not Identified	n/a	8	9	17
11	1085	10.12	Nonanal	266.15	8	9	17	•	•	•
12	1104	10.36	Not Identified	n/a	7	10	17
13	1140	10.77	(E)-2-Nonenal	67.68	5	8	13	•	•	•
14	1173	11.15	2-Decanone	19.47	5	4	9		•	•
15	1187	11.32	Decanal	60.63	7	5	12		•	•
16	1205	11.53	Dodecane	36.17	8	8	16		•	•
17	1256	12.08	Nonanoic acid	33.07	6	6	12		•	•
18	1331	12.88	2-(2-Butoxyethoxy)ethyl acetate	63.26	7	5	12		•	•
19	1337	12.94	(E)-2-Undecenal	16.74	4	5	9		•	•
20	1479	14.35	(S)-Germacrene D	0.67	7	7	14			•
21	1723	16.52	Not Identified	n/a	4	3	7

Peaks identified tentatively by coupled gas chromatography–mass spectrometry (GC–MS), confirmed by peak enhancement with authentic chemical standards and quantified at 100% by multiple point external standards. One microliter is equivalent to 33.6 hours of bed bug-exposed paper air entrainment (BBEXPH).
Number of responses by electroantennogram (EAG) by males and females and the composition of the three synthetic blends (SB) with 6 (SB-6), 16 (SB-16), and 18 (SB-18) GC-EAG-active chemicals tested is provided (black circles indicate chemicals present in each synthetic blend). The retention index of GC-EAG-active peak 1 was not calculable (n/c) as the alkane range did not extend this far into the trace on the HP-1 column.

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