Figure 2
N-t cleavage of Reelin decreased in the postnatal brain of the PA-DV KI mice. (A,C) Western blotting analysis of the indicated regions of the brain of WT (+/+), PA-DV heterozygous (+/KI), or homozygous (KI/KI) mice at P7 (A) or 2-month-old (C) using anti-Reelin G10 (top), anti-Dab1 (middle), and anti-β-actin (bottom) antibodies. Each lane represents a sample from an independent mouse of the indicated genotype. The position of each protein is indicated by the arrow. The positions of the molecular mass markers (kDa) are shown on the right side of the panels. (B,D) Quantification of Reelin FL, NR6, and NR2 of the indicated regions of the brain of WT (+/+), PA-DV heterozygous (+/KI), or homozygous (KI/KI) mice at P7 (B) or 2-month-old (D). White, light-gray, and black bars indicate the amount of each protein in the +/+, +/KI, and KI/KI mice, respectively. (E,F) Quantification of Dab1. of the indicated regions of the brain of WT (+/+), PA-DV heterozygous (+/KI), or homozygous (KI/KI) mice at P7 (E) or 2-month-old (F). White, light-gray, and black bars indicate the amount of Dab1 protein in the +/+, +/KI, and KI/KI mice, respectively. n = 8. CC: cerebral cortex, Hip.: hippocampus, Cb: Cerebellum. The data are shown as the mean ± SEM and were analyzed by One-way ANOVA followed by the Tukey-Kramer ad hoc test (B,E) or two-tailed Student’s t-test (D,F). n = 6 (B,E), n = 8 for WT mice and n = 10 for PA-DV homozygous mice (D,F). *p < 0.05, ***p < 0.001, and ****p < 0.0001. NS, not significant (p ≥ 0.05).
