Figure 3
The structures of the cerebral cortex and cerebellum are normal in PA-DV KI mice. (A–F) Immunohistochemistry of coronal brain sections of the indicated mice at E18.5. Anti-Reelin AF3820 (A,B), anti-Brn1 (green, C,D), anti-Ctip2 (green, E,F), and anti-Tbr1 (red, E,F) antibodies were used. Nuclei were stained using Hoechst 33342. Scale bar, 100 µm. (G–J) Immunohistochemistry of coronal brain sections of the indicated mice at P2. Anti-Reelin AF3820 (G,H) and anti-NeuN (green, I,J) antibodies were used. Nuclei were stained using Hoechst 33342 (I,J). Arrows indicate primitive cortical zone (I,J). Scale bar, 100 µm. (K–R) Immunohistochemistry of coronal brain sections of the indicated mice at P7. Anti-Reelin AF3820 (K,L), anti-Ctip2 (yellow, M,N), anti-Cux1 (O,P), and anti-Tbr1 (Q,R) antibodies were used. Nuclei were stained using Hoechst 33342. Scale bars, 100 µm (K,L,O–R), 300 µm (M,N). (S–V) Immunohistochemical analysis of the sagittal section of the cerebellum of mice at P7 (S,T) or 2 months (U,V) using anti-inositol-1,4,5-trisphosphate receptor type 1 (IP3R1), a marker of Purkinje cells. Nuclei were stained using Hoechst 33342 (blue). Scale bars, 300 µm (S,T), 1 mm (U,V).
