Figure 4
Excess Reelin signaling in the hippocampus causes ectopic neuronal localization in PA-DV KI mice. (A,B) Coronal sections of the hippocampus at P7 were immunostained with anti-Ctip2 (green) and anti-Reelin AF3820 (red) antibodies. Nuclei were stained using Hoechst 33342 (blue). Ectopic neurons were observed in the hippocampus of KI/KI mice (A, yellow box) but much less in that of KI/− mice (B). Scale bar, 300 µm. (C,D) Western blotting of hippocampus lysates from KI/KI and KI/− mice at P7 using anti-Reelin antibody G10 (C). Ponceau S staining showed approximately equal amounts of protein in each lane (D). The positions of full-length Reelin (FL) and the cleaved fragments (NR6, NR2) are indicated by the arrows in C. The positions of the molecular mass markers (kDa) are shown on the right. (E,F) Western blotting of hippocampus lysates from four independent KI/KI and KI/− mice at P7 using anti-Dab1 (top) and anti-β-actin (bottom) antibodies (E). The positions of the molecular mass markers (kDa) are shown on the right. Quantification of the blots is presented in (F). (G,H) Quantification of the position of Ctip2+ cells in the hippocampus. Scale bar, 100 µm. Data are presented as the mean ± SEM and analyzed by two-tailed Student’s t-test (F), or One-way ANOVA followed by the Tukey-Kramer ad hoc test (G). n = 6. *p < 0.05, **p < 0.01, ***p < 0.001.
