Figure 4
Doxycycline has no effect on cellular ATP levels synthesized through glycolysis or oxidative phosphorylation. Fibroblast, A549 and A549 ρ0 cultures were treated with vehicle (Veh) or doxycycline (DC) for 5 days. To determine the contribution of glycolysis and oxidative phosphorylation to the cellular ATP pool, cultures were subsequently left untreated or were treated with the oxidative phosphorylation inhibitor oligomycin A (Oligo) and/or the glycolysis inhibitor 2-deoxyglucose (2DG) directly prior to the ATP assays. Bars represent the mean cellular ATP levels (n = 4). Error bars indicate standard deviations. Asterisks denote statistically significant differences (p < 0.05).
