Figure 1

Transcriptional dynamics of Hippo effectors in NSCs, BPs, NBNs from RNA sequencing data. (a) Schematic representation of mouse developing cortex. NSCs reside in the VZ, with long processes extending from apical to basal surface. NSCs are labelled by Hes5::GFP. BPs express high levels of Tbr2::GFP (Tbr2::GFP^bright) and NBNs express low levels of Tbr2::GFP (Tbr2::GFP^dim) expression. (b) Experimental paradigm used. 3–4 RNA samples extracted from FAC-sorted GFP⁺ NSCs, BPs and NBN populations each day during development, from biological replicates, following the time-course (E10.5 to PN), through phases of expansion, neurogenesis and gliogenesis. cDNA libraries were prepared and Next-Generation RNA-sequencing performed. (c) Expression profiles of Hippo signaling effectors; receptors Fat1 and Crb2, ligands Dchs1, CD44, co-activators Yap1, Taz, transcription factors Tead1, Tead2, Tead3 in NSCs, BPs and NBNs show dynamics during corticogenesis in these populations. Y-axis: mRNA level expressed as log2 TPM (transcripts per million). Also see Figs. S1 and S2. NSCs- Neural stem cells, BPs- Basal progenitors, NBNs- Newborn neurons, VZ - ventricular zone, SVZ - subventricular zone, IZ - intermediate zone, CP - cortical plate, E - Embryonic day, PN - postnatal day 1.