Figure 6 | Scientific Reports

Figure 6

From: Seeking high-priority mutations enabling successful antibody-breeding: systematic analysis of a mutant that gained over 100-fold enhanced affinity

Figure 6

Protein ribbon structures for (A) scFv#4mut and (B) scFv#WT were constructed using the SWISS-MODEL Protein Modelling Server50, and their conformations when docked to E2 were predicted using SwissDock51. Three different views observed from different angles are shown. In the ribbon representation of the scFv backbones, CDR H1 (yellow), H2 (orange), H3 (magenta), L1 (dark blue), L2 (light green), and L3 (light blue) are represented with β-sheet structures (bold gray arrows). The introduced amino acids after the substitutions are shown in orange. The backbone of the E2 molecule is shown in light purple. Image generated with PyMOL52.

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