Figure 9

Accumulation of viral RNA in the foot processes of differentiated neurons. Live cell imaging (objective lens 20×) in lambda mode (spectral imaging) of TE (control) and TEds10Br-infected dAP7 cells (MOI = 20, 24 h after infection) plus DFHBI-1T. Viral RNA (green – Broccoli-DFHBI-1T) accumulated at filopodial tips (red arrow heads) in contact with an uninfected cell (yellow autofluorescence). Scale bar = 100 μm.