Figure 3

Reduced oxidative stress in Jdp2-KO GCPs. (a) ROS levels in WT and Jdp2-KO GCPs were measured using the ROS-Glo H₂O₂ assay described in Materials and Methods. The intracellular GSH level (b) and GSH/GSSG ratio (c) were measured and calculated as described in Materials and Methods. (d) ROS levels in WT and Jdp2-KO GCPs in the presence or absence of H₂O₂ (100 μM) for 2 h before harvesting cells for 24 h. (e) Levels of 8-oxo-dGuo in WT and Jdp2-KO GCPs in the presence or absence of H₂O₂ (100 μM) for 2 h before harvesting cells for 24 h. (f) MDA levels in the presence or absence of H₂O₂ (100 µM) for 2 h before the cultivation of cells for 24 h. (g) GSH/GSSG ratio in the presence or absence of H₂O₂ (100 μM) for 2 h before the cultivation of cells for 24 h. Data are presented as the mean ± SEM (n = 3); *p < 0.05.