Figure 1
From: Insulin activates hepatic Wnt/β-catenin signaling through stearoyl-CoA desaturase 1 and Porcupine
Wnt/β-catenin pathway activity is activated by refeeding in mouse liver. (a) Mice infected with Adv-TRE-Luc and Adv-RSV-β-gal were studied 4 days later in fasted or refed state as described in “Methods”. In vivo imaging of hepatic TRE-Luciferase activity (left panel) and quantification of luciferase activity (right panel) were performed. Results are expressed as percent of the ratio firefly luciferase/β-galactosidase. (b) Plasma insulin and blood glucose concentrations were measured in fasted and refed conditions. (c,d) Western blot analysis of liver protein expression in lysates from fasted and refed mice (c) and quantification of active-to-total β-catenin in liver of fasted and refed mice. (d) β-Actin antibody was used as a loading control (n = 7/group). (e,f) RT-qPCR analysis of Wnt target genes (GS and Lect2) (e) and of lipogenic gene (Fasn and Scd1) (f) expression in liver of fasted and refed mice. Results are the mean ± SEM (n = 5–7/group). **p < 0.01, ***p < 0.001 for refed vs fasted mice.
