Figure 4
From: Insulin activates hepatic Wnt/β-catenin signaling through stearoyl-CoA desaturase 1 and Porcupine
Porcupine is implicated in insulin-induced hepatic Wnt/β-catenin pathway activation. (a) TRE-Luc-expressing primary hepatocytes were infected with PORCNi or USi adenoviruses and incubated with either BSA (0.6%), palmitoleate (200 μM) for 24 h. Luciferase assays were performed and results are expressed as percent of the ratio firefly luciferase/β-galactosidase. Results are the mean ± SEM (n = 3). *p < 0.05, **p < 0.01 vs BSA; #p < 0.05 vs palmitoleate + USi. (b) TRE-Luc-expressing primary hepatocytes were infected with USi + GFP, SCD1 + USi or SCD1 + PORCNi adenoviruses and incubated with G25 or G25 plus insulin for 24 h. Luciferase assays were performed and results are expressed as percent of the ratio of firefly luciferase/β-galactosidase (n = 3). *p < 0.05 vs G25; ##p < 0.01 vs G25 + ins and $p < 0.05 vs SCD1 + USi. (c,d) TRE-Luc-expressing primary hepatocytes were infected with PORCNi or USi and incubated in G25 or G25 + ins for 24 h. (c) Luciferase assays and (d) RT-qPCR analysis of PORCN, GS and Lect2 gene expression were performed. Results in c and d are the mean ± SEM (n = 3–4). *p < 0.05, **p < 0.01, ***p < 0.001 vs G25; #p < 0.05, ##p < 0.01, ###p < 0.001 vs G25 + ins.
