Figure 4 | Scientific Reports

Figure 4

From: Piscidin-1 Induces Apoptosis via Mitochondrial Reactive Oxygen Species-Regulated Mitochondrial Dysfunction in Human Osteosarcoma Cells

Figure 4

Effect of piscidin-1 on mitochondrial function and respiration in the MG63 cell line. (A) The OCR was measured before and after adding pharmacological agents to living cells. Three measurements were taken and averaged to provide reliable data at the start, followed by sequential and continuous injections of oligomycin, FCCP, and antimycin A/rotenone. These analyses measured the basic parameters of basal respiration, ATP production, proton leak, maximum respiration, spare respiratory capacity, and non-mitochondrial respiration. (B) OCR value and time-course curve plot. The parameters quantified and analyzed in MG63 cells treated with 0, 0.1, 1, 5, and 10 μM piscidin-1 were the (C) basal respiration OCR, (D) ATP production (couple respiration), (E) proton leak, (F) maximal respiration, (G) spare respiratory capacity, and (H) non-mitochondrial respiration. OCR values were quantified by normalizing the cell protein concentration. The results are expressed as the mean ± SEM of three independent experiments. Significance was determined by Student’s t-test; *p < 0.05; **p < 0.01.

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