Figure 5

Effect of piscidin-1 on the expression levels of mitochondrial complex I–V protein and ATP production in human MG63 cells. (A) Cells treated with 0, 0.1, 1, 5, and 10 μM piscidin-1 for 24 h. The proteins of total cellular lysates were analyzed using Western blot analysis. The profile shows the effects of piscidin-1 treatment on the expression levels of complex I (NDUFB8), complex II (SDHB), complex III (UQCRC2), complex IV (COX II), complex V (ATP5A), and β-actin (as an internal control). The groupings were cropped from different gels subjected to identical conditions. Full blots are shown in the Supplementary Information, Fig. S6. The complex I subunit (B), complex II subunit (C), complex III subunit (D), complex IV subunit (E), and complex V subunit (F) protein levels were quantified and normalized to the β-actin level. (G) The effects of the total ATP production in MG63 cells treated with various concentrations of piscidin-1 for 24 h were measured using an ATP fluorescence kit. The results are expressed as the mean ± SEM of three independent experiments. Significance was determined using Student’s t-test; *p < 0.05; **p < 0.01.