Figure 7 | Scientific Reports

Figure 7

From: Piscidin-1 Induces Apoptosis via Mitochondrial Reactive Oxygen Species-Regulated Mitochondrial Dysfunction in Human Osteosarcoma Cells

Figure 7

Pretreatment with mitoTempo partially reversed the mtROS accumulation, MTP collapse, and apoptosis induced by piscidin-1 in MG63 cells. (A) Histogram of the fold changes after different treatment conditions compared with that in the control. (B) Flow cytometry histogram analyses of MTP after treatment with 10 μM piscidin-1, 5 μM MitoTempo, or both for 24 h. The loss of MTP (%) compared to untreated cells. (C) Annexin V/PI staining and flow cytometry dot analysis of MG63 cells after incubation with 5 μM mitoTempo for 2 h and treated with or without 10 μM piscidin-1 for an additional 24 h. (D) Quantification of apoptosis. The results are expressed as the mean ± SEM of three independent experiments. Significance was determined by Student’s t-test. **p < 0.01 versus control; #p < 0.05 versus piscidin-1 group.

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