Figure 4
From: Participation of Intussusceptive Angiogenesis in the Morphogenesis of Lobular Capillary Hemangioma
Perforations of interendothelial contacts and loop fragmentation into capillary-sized vessels. (A,B) An interendothelial contact (A, arrows) and its perforation (B, arrows) are shown in two sections obtained from serial semithin sections. Note the presence of neighbouring interstitial cells in the perforated zone. (C–G) Images in confocal microscopy with double immunofluorescent label (CD34 and collagen I, DAPI: blue) demonstrating the appearance and disappearance of a pillar (arrows), with an endothelial cover (green) and a core with collagen I (red). (H–J) Images showing loop fragmentation in several capillary-sized vessels after perforation of interendothelial contacts. Note that the loops arise from venules (v) and that the capillary-sized vessels are arranged in a linear arciform path reminiscent of that of loops. (A,B) Semithin section stained with Toluidine Blue. (C–G) Immunofluorescent label with CD34, collagen I and DAPI. (H–J) Sections double-stained with anti-CD34 (brown) and anti-αSMA (red). Bar: (A,B) 8 µm; (C–G) 30 µm; (H–J) 35 µm.
