Figure 3
Expression of miR-299 reduced tumor growth in xenograft models. C4-2B-299 cells were used to establish tumors in the flank of NSG mice. (A) Progression of tumor growth upon induction or no induction of miR-299 expression in the tumors. Mice were injected with C4-2B-299 cells and induced with feed containing doxycycline (dox-feed) or regular feed. Tumor growth was monitored for 28 days post induction by tumor volume measurement. Mice receiving the dox-feed showed a slower tumor growth compared to the control group of mice. (B) Average tumor volume showing a significant reduction in overall tumor volume in mice received dox-feed compared to the mice received regular feed at the time of euthanasia. Data show mean ± SD of tumor volumes of 5mice/group. (C) Mice receiving the dox-feed also were alive for a significantly longer time based on the time elapsed to reach the specified 1.5 cm3 tumor volume required for euthanization compared to the uninduced control group. (D,E) Quantitative RT-PCR showing increased expression of miR-299-3p (D) and reduced expression of AR (E) in tumors from mice that received dox-feed compared to the regular feed. Data show mean ± SD of three tumors from each group. (F) Quantification of Ki67+ cells in tumors from mice that received dox-feed compared to the regular feed. Data show percentage of Ki67+ cells in three tumors from each group.
