Figure 2
From: Selective USP7 inhibition elicits cancer cell killing through a p53-dependent mechanism
XL177A is a potent USP7 inhibitor and p53 stabilizer in cyto. (a) Western blots showing USP7 labeling by HA-Ub-VS after 6-hour cell treatment with XL177A or XL177B. (b) Densitometry from A (n = 2 experimental replicates, error bars = SD). (c) Whole cell lysate Western blots of MCF7 cells after 2-hr treatment with XL177A or XL177B (n = 2 experimental replicates). Full blots are presented in Fig. S14. (d) Whole cell lysate Western blots of MCF7 cells after 16-hr treatment with XL177A or XL177B (n = 2). (d) Quantitative real-time PCR of MCF7 cells treated for 24 hours with 1 μM XL177A or XL177B (n = 2 experimental replicates with 3 technical replicates each, error bars = SEM). E) Cell cycle analysis based on propidium iodide staining of MCF7 cells after 24-hr treatment with 1 μM XL177A or XL177B (n = 3 experimental replicates, error bars = SD).
