Figure 4
From: Selective USP7 inhibition elicits cancer cell killing through a p53-dependent mechanism
XL177A is selective for USP7. (a) Remaining activity of 41 purified recombinant DUBs against Ub-Rho after 15-minute pre-treatment with XL177A (n = 1). (b) Ratio of Bio-Ub-PA/VME labeling for 60 DUBs in HEK293AD lysate between samples pre-treated for 5 hours with DMSO v. 1 μM XL177A (n = 2 experimental replicates with two technical replicates, reported values are medians of all replicates). (c) Ratio of XL177A-DTB labeling for 566 proteins in HEK293AD lysate between samples pre-treated for 5 hours with DMSO v. 1 μM XL177A (n = 3 experimental replicates with two technical replicates, reported values are medians of all replicates).
