Figure 5
From: Selective USP7 inhibition elicits cancer cell killing through a p53-dependent mechanism
XL177A activates p53 signaling transcriptome-wide. (a) Heat map showing hierarchical clustering of transcriptional profiles from MCF7 cells treated for 24 hours with 0.1 or 1 μM XL177A, 0.1 or 1 μM XL177B, or 1 or 10 μM Nutlin-3A. (b) Volcano plots of genes enriched or depleted after 24-hr treatment with 1 μM XL177A. (c) Expression levels of all genes (clear) or direct p53 target genes (filled) from MCF7 cells treated for 24 hours with 1 μM XL177A (adjusted p < 0.0001), Nutlin-3A (adjusted p < 0.0001), or XL177B (ns). Box: inter-quartile range; Whiskers: gens within 3 SD of mean. (d) Top 5 datasets enriched in the set of genes significantly up- or down-regulated by 1 μM XL177A treatment and their ranking in the set of genes up- or down-regulated by Nutlin-3A. (e,f) Waterfall plot of all detected transcripts from 1 μM XL177A ranked by normalized expression score and compared to DREAM complex targets (e) or direct p53 targets (f).
